Gram staining
Happy sunny morning!!!
I came the busy lab in Saturday morning and did few gram staining experiments for caeni 2 and caeni 3. Both of them are Gram positive bacteria.
Fluorescent gram staining protocol v1
Slides were loaded into a BioTek slide in both a coverslip down Optimal exposure settings were determined for each objective using a positive control smear and then applied to all images for both positive and negative controls. Our group used microscope BioTek to do gram staining protocol with sample was CF594 (red color/cell wall) and Dapi (Blue color/ Nuclei) With + DAPI 377/477 1225100 Rev J - Blue + GFP 469/526 1225101 Rev K- green + TEXAS 586/647 125102 Rev J - Red I very like this new technique, and I think it is more exactly than normal gram staining technique I have used before. I hope I will do it few more time in future so I can use to do this new method.
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