Using a Micropipette
1. What is micropipette?
The micropipette is used to transfer small amounts (< 1 ml) of liquids. The scales on micropipettes are in microliters (1000 !l = 1 ml). The brand of micropipettes we will be using is made by Rainin and called a "Pipetman". These are very expensive, delicate instruments costing $250-300 apiece. We have four sizes identified by the number on the round button on the plunger. The value is the maximum volume in microliters that can be transferred with that size pipette. They are used in conjunction with disposable sterile plastic tips.
2. The limits of micropipette?
The limits are:
- P10: 1.0 - 10.0 μm
- P20: 2.0 - 20.0 μm
- P200: 20 - 200 μm
- P1000: 200 - 1000 μm
Look at the front face of the pipet and you will see a window with three digits inside. The diagram below shows the MAXIMUM value that can or should be dialed in on each size pipet. To exceed these values will put the pipet out of calibration. Beside each "window" below is the numbers place it represents. Please take the time to learn how to read them so as to avoid damaging them by dialing values out of their range.
The micropipette is used to transfer small amounts (< 1 ml) of liquids. The scales on micropipettes are in microliters (1000 !l = 1 ml). The brand of micropipettes we will be using is made by Rainin and called a "Pipetman". These are very expensive, delicate instruments costing $250-300 apiece. We have four sizes identified by the number on the round button on the plunger. The value is the maximum volume in microliters that can be transferred with that size pipette. They are used in conjunction with disposable sterile plastic tips.
2. The limits of micropipette?
The limits are:
- P10: 1.0 - 10.0 μm
- P20: 2.0 - 20.0 μm
- P200: 20 - 200 μm
- P1000: 200 - 1000 μm
Look at the front face of the pipet and you will see a window with three digits inside. The diagram below shows the MAXIMUM value that can or should be dialed in on each size pipet. To exceed these values will put the pipet out of calibration. Beside each "window" below is the numbers place it represents. Please take the time to learn how to read them so as to avoid damaging them by dialing values out of their range.
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