Isolate high quality DNA from microbial cultures
I love working with DNA field. We used the DNeasy® UltraClean® Microbial Kit for DNA isolation on radioduran (1), metalli (2), and misoensis (3) with detail procedure: We must know important points before starting: - If Solution SL has precipitated, heat at 55°C for 5–10 min. - We must shake to mix Solution SB before use. Procedure: 1. Add 1.8 ml of microbial (bacteria, yeast) culture to a 2 ml Collection Tube (provided) and centrifuge at 10,000 x g for 30 s at room temperature. Decant the supernatant and spin the tubes again at 10,000 x g for 30 s at room temperature. Completely remove the supernatant with a pipette tip. Note: Depending on the type of microbial culture, it may be necessary to centrifuge longer than 30 s. 2. Resuspend the cell pellet in 300 µl of PowerBead Solution and gently vortex to mix. Transfer resuspended cells to a PowerBead Tube. 3. Add 50 µl o...