S-STEM Scholar Dung Le's Blog

PGLO transformation 1. Label two B12 competant (able to take DNA) Ecoli cell tubes and (-) PGLO, (+) PGLO and place on ice. 2. Transfer 250  µm of cold transformation solution CaCl2 into each tube 3. Place tubes on crushed ice 4. Using pipet 10  µL of PGLO plamid (DNA) into + PLGO tube. 5. Incubate tubes onn ice for 10 mins 6. Lable 4 agar plates on the bottom  (1)  LB with  (-) PGLO (2) LB/Amplara  with  (-) PGLO (3) LB/Amplara  (+) PGLO (4) LB/Amplara/Ara    (+) PGLO Note: We must test the result that (1) Cell can grow with lots of growth (2) No growth (3) Growth not much (4) Growth/ Glow (Has a light) 7. Heat shock using both for   (-) PGLO, (+) PGLO tubes into water bath at 42  degrees celsius for 50 sec. Then place back on ice in 2 mins. 8. Remove from ice and rack. Add 250  µm LB broth into tubes and incubate at room temp for 10 min 9. Grenly flick closed...

Deinococcus radiodurans I. Introduction Deinococcus radiodurans  is an extremophilic bacterium, one of the most radiation-resistant organisms known. It can survive cold, dehydration, vacuum, and acid and is therefore known as a polyexremophile  and has been listed as the world's toughest bacterium in The Guinness Book of World Records. Deinococcus radiodurans was discovered in 1956 by an American researcher when he intended to sterilize beef canned foods by irradiating an intense gamma radiation. He was surprised to note that bacteria did not die below this radiation level. Deinococcus radiodurans is able to withstand radiation levels hundreds of times higher than lethal doses. The gene of Deinococcus radiodurans has a ring-shaped structure that breaks into thousands of pieces. The researchers noted that the cells appear to die in about an hour and a half. But three hours after the radiation, its D...